Tris (hydroxymethyl) aminomethane

☑ Tris tsis yog tsuas yog siv los ua cov kuab tshuaj rau nucleic acids thiab cov proteins, tab sis kuj muaj ntau yam tseem ceeb siv.Tris tau siv rau cov protein siv lead ua kev loj hlob nyob rau hauv cov pH sib txawv.
☑ Lub zog qis ionic ntawm Tris tsis tuaj yeem siv los tsim cov fiber ntau nruab nrab ntawm lamin hauv C. elegans.
☑ Tris kuj yog ib qho tseem ceeb ntawm cov protein electrophoresis tsis.
☑ Tsis tas li ntawd, Tris yog qhov nruab nrab rau kev npaj cov surfactants, vulcanization accelerators thiab qee cov tshuaj.Tris kuj tseem siv los ua tus qauv titration.
☑ Trimethylaminomethane yog dav siv nyob rau hauv mob metabolic thiab ua pa acidemia.Nws yog ib qho alkaline tsis thiab muaj cov nyhuv tsis zoo ntawm metabolic acidosis thiab cov tshuaj tiv thaiv enzyme.
☑Tris feem ntau yog siv los ua cov khoom siv roj ntsha, thiab nws tus nqi pH yog 6.8, 7.4, 8.0, 8.8.Nws cov qauv qauv, pH tus nqi hloov pauv nrog qhov kub thiab txias.Feem ntau hais lus, tus nqi pH txo qis los ntawm 0.03 thaum qhov kub nce los ntawm ib qho degree.Tris yog dav siv nyob rau hauv kev npaj cov tshuaj tsis nyob rau hauv biochemistry thiab molecular biology thwmsim.Piv txwv li, ob qho tib si TAE thiab tbe buffers (siv rau nucleic acid dissolution) feem ntau siv hauv kev sim biochemical xav tau Tris.Vim nws muaj cov amino pawg, nws tuaj yeem hnov ​​​​mob nrog aldehydes.
☑ Tris yog lub hauv paus tsis muaj zog, thiab nws PKA yog 8.1 ntawm chav tsev kub (25 ℃).Raws li txoj kev xav tsis, qhov tsis zoo ntawm Tris tsis yog nyob nruab nrab ntawm 7.0 thiab 9.2.Tus nqi pH ntawm Tris puag aqueous tov yog hais txog 10.5.Feem ntau, hydrochloric acid yog ntxiv los kho tus nqi pH rau tus nqi xav tau kom tau txais cov tshuaj tsis haum nrog tus nqi pH no.Nyob rau tib lub sijhawm, peb yuav tsum them sai sai rau qhov ntsuas kub ntawm pKa ntawm Tris.Vim tias Tris tsis yog cov tshuaj tsis muaj zog alkaline, DNA yuav raug deprotonated hauv cov tshuaj no los txhim kho nws cov solubility.Tib neeg feem ntau ntxiv EDTA rau hauv Tris hydrochloric acid buffer los ua "Te buffer", uas yog siv rau DNA stabilization thiab cia.Yog hais tias cov kua qaub ntawm kev hloov pH tus nqi yog hloov los ntawm acetic acid, "Tae buffer" (Tris / acetate / EDTA) tau txais, thiab "tbe buffer" (Tris / borate / EDTA) yog tau los ntawm kev hloov nws nrog boric acid. .Ob qhov buffers no feem ntau yog siv rau hauv kev sim nucleic acid electrophoresis.